The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species
The expression of Beclin1 and LC3 in pancreatic cancer cells. (a) The expression of Beclin1 and LC3-II protein in BxPc-3, PANC-1, SW1990, AsPC-1, and MiaPaCa-2 cells was evaluated by Western blotting. (b-c) The expression of Beclin1 and LC3 mRNA level was estimated in 5 pancreatic cancer cell lines by qRT-PCR. The data are presented as Mean ± SD for three independent experiments. The bar graph shows the relative mRNA expression levels among the cell lines. Column: Mean; bar: SD.