The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species
Suppression of autophagic activity enhances Nrf2 expression and translocation of Nrf2 to the nucleus upon ROS stimulation in PANC-1 cells. (a) The expression of Beclin1 and LC3-II treated with or without 3-MA or CQ in the absence of ROS. (b) The expression of Nrf2, Beclin1, and LC3-II and also the level of p62 in pancreatic cancer cells under basal condition and upon ROS stimulation with or without 3-MA or CQ. (c) Immunofluorescence images of PANC-1 cells for Nrf2 under basal condition and upon ROS stimulation with or without 3-MA or CQ.