The Relevance of Nrf2 Pathway and Autophagy in Pancreatic Cancer Cells upon Stimulation of Reactive Oxygen Species

Figure 6

Silencing of Nrf2 in PANC-1 cells upon ROS stimulation promotes autolysosome formation. (a) PANC-1 cells were infected with the Ad-mRFP-GFP-LC3. Then, the cells were transfected with Nrf2 siRNA, treated with ROS stimulation, and observed by Live Cell Imaging Confocal Scanner System. (b) PANC-1 cells were infected with the Ad-mRFP-GFP-LC3. Then, the cells were transfected with NC siRNA, treated with ROS stimulation, and observed by Live Cell Imaging Confocal Scanner System. (c-d) Numbers of puncta in the same cell was determined when cells were under basal condition and upon ROS stimulation. Cells were counted in 10 random fields. The data of fold increase are presented as Mean ± SD for three independent experiments. Column: Mean; bar: SD.

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