Inhibition of Glucose-6-Phosphate Dehydrogenase Could Enhance 1,4-Benzoquinone-Induced Oxidative Damage in K562 Cells

Figure 5

Effect of BQ on DNA damage in G6PD control and G6PD inhibition cells. The cells were treated with 0, 10, and 20 μmol/L BQ for 24 h and then were analyzed under epifluorescence microscope. Data were expressed as mean ± SD with 50 cells in each group. Tail DNA% and Olive Tail Moment were analyzed through comet assay. (a) Tail DNA% = 100 × Tail DNA Intensity/Cell DNA Intensity. (b) Olive Tail Moment = Tail DNA%  × Tail Moment Length. (c) Representative photographs obtained through epifluorescence microscopy using an FITC filter. compared with 0 μmol/L group; compared with G6PD control.

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