Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation

Figure 4

High glucose culture increases ROS generation and cytokines release within BRL-3A hepatocytes. ((a), (b)) Intracellular ROS stained by 6-carboxy-2′7′-DCFH-DA (200x) and the fluorescence intensity was detected by fluorescence spectrophotometer. ((c), (d)) TNF-α and MCP-1 in the culture medium. versus 5.5 mM control group; versus 25 mM control group; versus 5.5 mM H/R group. H/R: hypoxia/reoxygenation; ROS: reactive oxygen species; TNF-α: tumor necrosis factor α; MCP-1: monocyte chemokine protein-1.

(a)

(b)

(c)

(d)