Hyperglycemia Aggravates Hepatic Ischemia Reperfusion Injury by Inducing Chronic Oxidative Stress and Inflammation

Figure 7

Hepatic ischemia reperfusion injury, oxidative stress, and inflammation in NAC or apocynin pretreated diabetic rats. ((a), (b)) Hepatic pathology sections (200x), pathological scores, and serum transferase. (c) Hepatic inflammatory cytokines levels. ((d), (e)) Nuclear 8-OHdG (400x) detected by IHC staining and IOD assessed by Image-J; (f) MDA levels; (g) 8-isoprostane levels by ELISA. Measurable data are expressed as mean ± SEM ( per group). Pathology scores are expressed as medium with interquartile range. versus sham group; versus sham group; versus sham group; versus HIRI group; versus HIRI group; versus HIRI group. HIRI: hepatic ischemia reperfusion injury; NAC: N-acetyl-L-cysteine; IHC: immunohistochemistry; 8-OHdG: 8-hydroxydeoxyguanosine; IOD: integrated optical intensity; MDA: malondialdehyde; TNF-α: tumor necrosis factor α; MCP-1: monocyte chemokine protein-1; IL-6: interleukin-6; ELISA: enzyme-linked immunosorbent assay; sham: sham operating group.

(a)

(b)

(c)

(d)

(e)

(f)

(g)