Oligonol Ameliorates CCl₄-Induced Liver Injury in Rats via the NF-Kappa B and MAPK Signaling Pathways

Table 3

Antioxidative activities of oligonol against the lipid peroxidation of rat liver homogenates induced by FeSO₄ and H₂O₂.

Substances	Concentration	Inhibition (%)	IC50
Oligonol (µg/mL)	10	47.52 ± 0.06	15.16
	50	62.01 ± 0.01
	100	74.74 ± 0.06
	500	93.02 ± 0.01
BHT (µM)	5	16.51 ± 0.04	15.01
	10	43.43 ± 0.03
	20	59.82 ± 0.01
	50	89.61 ± 0.05

Results are expressed as mean ± SE (). The reaction mixture was composed of the rat liver homogenate, 0.1 mM FeSO₄, 3 mM H₂O₂, and various concentrations of oligonol or BHT. After incubation at 37°C for 10 min, the amount of MDA formation was measured by the method of Buege and Aust. Inhibition (%) of MDA formation in oligonol or BHT was calculated based on the amount of MDA formation of the FeSO₄/H₂O₂-treated control after subtracting the normal.