High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster

Figure 1

Lifespan and survival curves and body weight due to a high-fat diet. Wild type Drosophila melanogaster (strain Harwich: both sexes) were placed on regular diet (RD) and high-fat diet (HFD) under controlled conditions. (a) Lifespan: the flies were counted daily until there were no more flies alive. Coconut oil (10% and 20%) in the diet caused a significant decrease in lifespan. Four sets of twenty-five flies were used for each diet condition. (b) Survival curve in seven days: the number of live and dead flies was counted every 24 h during seven days of the treatment and exposure of diets. Coconut oil (10% and 20%) in the diet caused a significant decrease in survival rate. Three sets of twenty flies were used for each diet condition. (c) Body weight was evaluated at first and seventh days after the exposure to the different diets (data expressed in percentage of the 0% coconut oil group, RD). Three sets of twenty flies were used for each diet condition (mean ± standard deviation). in relation to RD group.

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