High-Fat Diet Induces Oxidative Stress and MPK2 and HSP83 Gene Expression in Drosophila melanogaster

Figure 4

Effects on oxidative stress parameters in flies treated for seven days with a regular diet (RD) and a high-fat diet (HFD). After seven days on the diets, flies were homogenized and the supernatant was used for various analyses of stress markers and the activity of antioxidant enzymes. (a) Showing the DCF-DA intensity of fluorescence in total flies homogenate. Three sets of twenty flies were used for each diet condition. (b) End products of lipid peroxidation determined by TBARS assay in total flies homogenate, expressed in MDA nmoles/mg of protein. Three sets of twenty flies were analyzed for each diet condition. (c) Catalase (CAT) activity and (d) superoxide dismutase (SOD) activity in total flies homogenate. Three sets of twenty flies were analyzed for each diet condition. Data are expressed as a mean ± standard deviation in mU/mg protein. indicates a significant difference in relation to RD ().

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