Parishin from Gastrodia elata Extends the Lifespan of Yeast via Regulation of Sir2/Uth1/TOR Signaling Pathway

Figure 4

Effects of parishin on the replicative lifespan of sod1 (a), sod2 (b), uth1 (c), and skn7 (d) mutant yeast strains with a K6001 background. The daughter cells of 40 microcolonies of each experiment were counted. The assay was repeated at least thrice. The results were displayed as mean ± SEM. The average lifespan of untreated K6001 was generations; RES at 10 μM, ; and parishin at 10 μM, . (a) Δsod1 was ; RES at 10 μM, and; parishin at 10 μM, . (b) Δsod2 was ; RES at 10 μM, ; and parishin at 10 μM, . (c) Δuth1 was ; RES at 10 μM, ; and parishin at 10 μM, . (d) Δskn7 was ; RES at 10 μM, ; and parishin at 10 μM, . Δsod1, Δsod2, Δuth1, and Δskn7 represent sod1, sod2, uth1, and skn7 mutant yeast strain with a K6001 background, respectively. indicates significant difference compared with untreated K6001 ().

(a)

(b)

(c)

(d)