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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 4139851, 7 pages
http://dx.doi.org/10.1155/2016/4139851
Research Article

Tempol, a Membrane-Permeable Radical Scavenger, Exhibits Anti-Inflammatory and Cardioprotective Effects in the Cerulein-Induced Pancreatitis Rat Model

1Chair and Department of Pathophysiology, Medical University of Lublin, 20-090 Lublin, Poland
2Chair and Department of Clinical Pathomorphology, Medical University of Lublin, 20-090 Lublin, Poland
3Department of Pharmacology, Medical University of Lublin, 20-093 Lublin, Poland
4Chair and Department of Vascular Surgery and Angiology, Medical University of Lublin, 20-081 Lublin, Poland
5Department of Pharmacology, University of Life Sciences in Lublin, 20-033 Lublin, Poland

Received 4 June 2015; Revised 30 August 2015; Accepted 31 August 2015

Academic Editor: Swaran J. S. Flora

Copyright © 2016 Andrzej Marciniak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

To date, it remains unclear whether mild form of acute pancreatitis (AP) may cause myocardial damage which may be asymptomatic for a long time. Pathogenesis of AP-related cardiac injury may be attributed in part to ROS/RNS overproduction. The aim of the present study was to evaluate the oxidative stress changes in both the pancreas and the heart and to estimate the protective effects of 1-oxyl-2,2,6,6-tetramethyl-4-hydroxypiperidine (tempol) at the early phase of AP. Cerulein-induced AP led to the development of acute edematous pancreatitis with a significant decrease in the level of sulfhydryl (–SH) groups (oxidation marker) both in heart and in pancreatic tissues as well as a substantial increase in plasma creatine kinase isoenzyme (CK-MB) activity (marker of the heart muscle lesion) which confirmed the role of oxidative stress in the pathogenesis of cardiac damage. The tempol treatment significantly reduced the intensity of inflammation and oxidative damage and decreased the morphological evidence of pancreas injury at early AP stages. Moreover, it markedly attenuated AP-induced cardiac damage revealed by normalization of the –SH group levels and CK-MB activity. On the basis of these studies, it is possible to conclude that tempol has a profound protective effect against cardiac and pancreatic damage induced by AP.