Iron-Mediated Lysosomal Membrane Permeabilization in Ethanol-Induced Hepatic Oxidative Damage and Apoptosis: Protective Effects of Quercetin

Figure 2

Quercetin extenuated hepatocytes apoptosis elicited by ethanol. TUNEL staining was used to assay hepatocytes apoptosis (a). The TUNEL-positive nucleus showed brown (black arrow) and the negative nucleus showed blue (blue arrow). (b) Quantitation of data obtained in (a) by using the IPP image analysis software. The activation of caspases-3 was analyzed by Western blotting (c, ) and quantified by Image J software (c). Blotting with anti-β-actin was used as a protein loading control. The activity of caspase-3 was determined by assay kit (Beyotime) and standardized by protein concentration measured by the Bio-Rad protein assay kit (d). Results were presented as mean ± SD or mean ± SE. C: control; E: ethanol; EQ: ethanol plus quercetin; ED: ethanol plus DFO; Q: quercetin; D: DFO. A: versus the control; B: versus the ethanol group.

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