Iron-Mediated Lysosomal Membrane Permeabilization in Ethanol-Induced Hepatic Oxidative Damage and Apoptosis: Protective Effects of Quercetin

Figure 5

Quercetin attenuated ethanol-induced lysosomal membrane permeabilization (LMP) and mitochondria membrane permeabilization (MMP). The alterations of LMP were assayed by AO staining with flow cytometry, as evident by decrease in FL3 red fluorescence and increase in FL1 green fluorescence (a and b). Cathepsin B reconstitution (c) and activity (d) were determined by Western blot and assay kit. The red mitochondrial fluorescence stained with TMRM of 10,000 cells per sample was determined by flow cytometry by using the FL3 channel (e). Cytochrome C release (f) was evaluated by Western blotting and the band densities were measured by Image J software (g, ). The blotting with GAPDH and VDAC1 was used as a protein loading control. C: control; E: ethanol; EQ: ethanol plus quercetin; ED: ethanol plus DFO; Q: quercetin; D: DFO.