NRF2 Regulates HER2 and HER3 Signaling Pathway to Modulate Sensitivity to Targeted Immunotherapies

Figure 3

Nrf2 activation leads to transcriptional induction of HER2 and HER3 in a concentration dependent manner. (a) SKOV3 cells exhibit higher basal transcription of both HER2 and HER3. PEO1 and SKOV3 cells were transfected with either empty PGL3 basic vector or 1 μg PGL3 basic vector with cloned 1.5 kb fragments of either HER2 (prHER2) or HER3 (prHER3) promoter driving the expression of luciferase gene. Cotransfection with 0.2 μg pRL-CMV plasmid was performed as an internal transfection control. (b) TBHQ causes transcriptional induction of HER2 and HER3 in a concentration dependent manner. PEO1 and SKOV3 cell lines were transfected in triplicate as in (a) but were treated with different concentrations of tBHQ as indicated for 4 h. Data shown are the means ± S.D. of triplicates, normalised to untreated (UT) controls and expressed as fold change with statistical significance determined by ONE WAY ANOVA followed by Tukey’s post hoc test (:  , :  , and :  ).

(a)

(b)