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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 4257498, 11 pages
http://dx.doi.org/10.1155/2016/4257498
Research Article

The Coadministration of N-Acetylcysteine Ameliorates the Effects of Arsenic Trioxide on the Male Mouse Genital System

1Department of Morphology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil
2Department of Pharmacology, Institute of Biosciences, Universidade Estadual Paulista (UNESP), 18618-970 Botucatu, SP, Brazil
3Department of Clinical Analyses, Toxicology and Food Sciences, School of Pharmaceutical Sciences, University of São Paulo (USP), 14040-903 Ribeirão Preto, SP, Brazil
4Department of Morphology, Stomatology, and Physiology, School of Dentistry, University of São Paulo (USP), 14040-903 Ribeirão Preto, SP, Brazil

Received 4 May 2015; Accepted 30 September 2015

Academic Editor: Joseph A. Adeyemi

Copyright © 2016 Raquel Frenedoso da Silva et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Arsenic trioxide (As2O3) has shown effectiveness in treatment of leukemia but is also associated with reproductive toxicity. Since remediation with N-acetylcysteine (NAC) may mitigate the adverse effects caused by exposure, we assessed the effects of As2O3 and its potential reversibility after exposure cessation or coadministration of NAC. Animals received 0.3 or 3.0 mg/Kg/day of As2O3 subcutaneously and 40 mM of NAC in tap water. As2O3 treatment impaired spermatogenesis and sperm motility and decreased seminal vesicle weight and testosterone serum levels; after suspension of treatment, these parameters remained altered. When NAC was administered, animals showed improvement in sperm parameters and seminal vesicle weight. In vitro epididymal contractility was increased in As2O3-treated animals. We concluded that As2O3 is toxic to the male mouse genital system by compromising sperm quality and quantity; these effects persisted even after suspension of the treatment. However, the coadministration of NAC ameliorates the harmful effects of the drug on the male genital system.