PTEN Activation by DNA Damage Induces Protective Autophagy in Response to Cucurbitacin B in Hepatocellular Carcinoma Cells

Figure 1

The cytotoxicity of Cuc B to BEL-7402 cells. Cells were treated with Cuc B for 6 h and the cell morphology was observed with microscopy (20) (a), the cell viability was determined by MTT (c), and the LDH release was determined (e). Cells () were treated with Cuc B for 6 h and then cultured for 2 weeks. The culture medium was replaced every 3 days. The colony was determined by staining with crystal violet and images were captured with a microscope (4) (b). Cells were treated with Cuc B (100 nM) for 6 h, 24 h, and 48 h and the cell viability was determined by MTT (d). Cuc B, Cucurbitacin B. .

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