PTEN Activation by DNA Damage Induces Protective Autophagy in Response to Cucurbitacin B in Hepatocellular Carcinoma Cells

Figure 2

Cuc B induced DNA damage response. Cells were treated with Cuc B for 6 h and the DNA damage was detected by comet assay (a) and the levels of γ-H₂AX (b), ATM, p-ATM, ATR, p-ATR (d), and Chk1, p-Chk1, Chk2, and p-Chk2 (e) were detected by Western blot. Cells were treated with Cuc B (100 nM) and the expression of γ-H₂AX was detected (c). Cells were pretreated with KU55933 (10 μM) (f) or caffeine (10 μM) (g) for 2 h followed by cotreatment with Cuc B (100 nM) for 6 h, and the protein expression was determined by Western blot. Cuc B, Cucurbitacin B.

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