PTEN Activation by DNA Damage Induces Protective Autophagy in Response to Cucurbitacin B in Hepatocellular Carcinoma Cells

Figure 3

Cuc B induced protective autophagy. Cells were treated with Cuc B for 6 h and stained with MDC (20) (a) and the protein expression was detected by Western blot (b and d). Cells were treated with Cuc B (100 nM) and the expression of LC3 was detected by Western blot (c). Cells were pretreated with 3-MA (2.5 mM) (e) or CQ (10 μM) (f) for 2 h and then cotreated with Cuc B for 6 h, and the cell viability was determined by MTT. Cuc B, Cucurbitacin B; MDC, monodansylcadaverine. ; .

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