PTEN Activation by DNA Damage Induces Protective Autophagy in Response to Cucurbitacin B in Hepatocellular Carcinoma Cells

Figure 4

Cuc B induced caspase-mediated apoptosis. Cells were treated with Cuc B for 6 h and apoptosis, protein expressions, and DNA condensation were determined by Annexin V/PI double staining (a), Western blot (b and c), and Hoechst 33342 staining (20) (d), respectively. Cuc B, Cucurbitacin B. .

(a)

(b)

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(d)