Apigenin Attenuates Oxidative Injury in ARPE-19 Cells thorough Activation of Nrf2 Pathway

Figure 2

Apigenin upregulates Nrf2 expression and promotes its nuclear translocation in ARPE-19 cells. (a) Real-time PCR analyses of Nrf2 mRNA expression in t-BHP-treated ARPE-19 cells. Significance: versus t-BHP. (b) Real-time PCR analyses of Nrf2 mRNA expression in ARPE-19 cells in the absence of t-BHP. Significance: versus control siRNA. (c) Western blot analyses of protein abundance of Nrf2 in cytoplasm and nucleus in t-BHP-treated ARPE-19 cells with quantification. Significance: versus control, versus t-BHP, and versus t-BHP. (d) Western blot analyses of protein abundance of Nrf2 in cytoplasm and nucleus in ARPE-19 cells in the absence of t-BHP with quantification. Significance: ^## versus control.

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