Acetaldehyde Induces Cytotoxicity of SH-SY5Y Cells via Inhibition of Akt Activation and Induction of Oxidative Stress

Figure 1

Effects of acetaldehyde on the cell viability and apoptosis of SH-SY5Y cells. Cells were incubated with various concentrations of acetaldehyde for 24 h. (a) Cell viability was determined with trypan blue assay. The number of cells in control group is set to 100%. (b) The cells were fixed and stained with Hoechst 33258. (c) SH-SY5Y cells were treated with different concentration of acetaldehyde for 24 h. Total RNA was isolated after the treatment and the expression of Bcl-2, Bcl-xL, and Bax genes was assessed by RT-PCR. The expression of GAPDH was used as an internal control. (d) Total lysates of cells were collected and the caspase 3 activities were determined. Data are expressed as the values of concentration of pNA. Values are means ± SD, . , significantly different from untreated cells ().

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