Acetaldehyde Induces Cytotoxicity of SH-SY5Y Cells via Inhibition of Akt Activation and Induction of Oxidative Stress

Figure 3

Effects of acetaldehyde on the activation of p38MAPK/ERK/JNK in SH-SY5Y cells. SH-SY5Y cells were treated with different concentrations of acetaldehyde for 24 h. Total cell lysates were collected and the protein levels of p38MAPK, phosho-p38MAPK (Thr180/Tyr182) (a), ERK (p44/p42) MAPK and phospho-ERK (Thr202/Tyr204) (b), and JNK and phospho-JNK (Thr183/Tyr185) (c) were determined by Western blot analyses. The intensities of the bands were quantified by densitometric analyses and normalized by the amount of p38MAPK, JNK, or ERK. Values are means ± SD from three independent experiments. , significantly different from control (); , significantly different from control ().

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