S-Propargyl-cysteine Exerts a Novel Protective Effect on Methionine and Choline Deficient Diet-Induced Fatty Liver via Akt/Nrf2/HO-1 Pathway

Figure 10

Induction of HO-1 by SPRC was critical for its antioxidative activity in HepG2 cells. (a) The cells were incubated with HO-1 siRNA or scrambled siRNA and then incubated with SPRC (50 μM) for 24 h, and the expression of HO-1 was detected by Western blot, and GAPDH was used as loading control; data shown are means ± SEM; compared with nontreated control; compared with HO-1 siRNA. Data were from at least three independent experiments, each performed in duplicate. (b) The cells were incubated with HO-1 siRNA or scrambled siRNA and then incubated with SPRC (50 μM) for 24 h. Representative images of ROS level via DHE fluorescence in HepG2 cells detected by a laser confocal microscope at the end of the treatment (original magnification, ×200). (c) Proposed cell signaling pathway for SPRC-induced p-Akt/Nrf2/HO-1-mediated cytoprotective effect. Green arrow presents stimulation and red bar indicates inhibition.

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