S-Propargyl-cysteine Exerts a Novel Protective Effect on Methionine and Choline Deficient Diet-Induced Fatty Liver via Akt/Nrf2/HO-1 Pathway

Figure 5

The effects of SPRC on cell viability, lipid contents, and ROS level in HepG2 cells. (a) HepG2 cells were incubated with different SPRC concentrations (1, 10, 50, and 100 μM) for 6 h and then stimulated with OA (1.5 mM) for 18 h. Cell viability was measured by CCK-8 assay at the end of the treatment. (b) The same treatment was done as the viability detection, and ROS level was measured by microplate reader. The data represents mean ± SD. compared with nontreated control; compared with OA-treated control. (c) Cells were incubated with or without PAG (2 mM) for 30 min followed by SPRC (50 μM) for 6 h. Thereafter, cells were then treated with OA (1.5 mM) for 18 h. After the treatment duration, cells were stained with oil red O dye. The representative images of cells were captured by microscope at ×200 magnification. (d) Cells were incubated with or without PAG (2 mM) for 30 min followed by SPRC (50 μM) for 6 h. Cells were then treated with OA (1.5 mM) for 18 h. Representative images of ROS level via DHE fluorescence in HepG2 cells detected by a laser confocal microscope at the end of the treatment. (e) Oil red O stained cells were measured at 510 nm wave length by using microplate reader. Data represent mean ± SEM of three independent experiments. (f) Quantitative analysis for ROS level in HepG2 cells. The data represents mean ± SD. compared with nontreated control; compared with OA-treated control; compared with OA plus PAG.

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