S-Propargyl-cysteine Exerts a Novel Protective Effect on Methionine and Choline Deficient Diet-Induced Fatty Liver via Akt/Nrf2/HO-1 Pathway

Figure 8

SPRC-induced Akt phosphorylation in OA-stimulated HepG2 cells. (a) Cells were incubated with indicated concentrations of SPRC (0, 1, 10, 25, 50, and 100 μM) for 30 min. Western blot analysis gave the related ratio of p-Akt/Akt. (b) Cells were treated with SPRC (50 μM) for indicated time periods. The densitometric analysis gave the related ratio of p-Akt/Akt. (c) Cells were only stimulated with OA (1.5 mM) for indicated time periods. Western blot analysis gave the related ratio of p-Akt/Akt. (d) Cells were preincubated with or without 50 μM SPRC for a period of indicated times before addition of 1.5 mM OA for another 15 min. The densitometric analysis gave the related ratio of p-Akt/Akt. (e) Cells were preincubated with 10 μM LY294002 or 2 mM PAG for 30 min before adding SPRC (50 μM) for 30 min and. subsequently, stimulated with OA for another 15 min. The densitometric analysis gave the related ratio of p-Akt/Akt. Data represent mean ± SEM of three independent experiments. compared with nontreated control; compared with OA-treated control; compared with OA plus PAG or LY294002.

(a)

(b)

(c)

(d)

(e)