Anti-Inflammatory Therapy Modulates Nrf2-Keap1 in Kidney from Rats with Diabetes
Table 1
General and biochemical parameters in the experimental groups at short- and long-term diabetes induction.
C
D
DMMF
7 days
30 days
7 days
30 days
7 days
30 days
Body weight (g)
282 ± 17.82
428 ± 18.18
294.9 ± 3.46
273.5 ± 7.28
288.8 ± 12.67
295.8 ± 9.53
Blood glucose (mg/dL)
85.7 ± 12.37
90.58 ± 14.27
420 ± 35
438 ± 28.27
390.0 ± 15.73
368.9 ± 17.85
Serum creatinine (mg/dL)
0.40 ± 0.08
0.45 ± 0.02
0.45 ± 0.01
0.43 ± 0.06
0.42 ± 0.02
0.48 ± 0.05
Diuresis (mL/24 hrs)
16.66 ± 2.4
17.8 ± 6.8
23.86 ± 2.29
39.68 ± 5.05
21.37 ± 1.2
27.95 ± 4.32
Glucosuria (mg/dL)
0 ± 0
0 ± 0
4817 ± 180.9
4791 ± 189.8
4302.50 ± 164.5
4377 ± 169.5
Total GFR (mL/min)
ND
1.31 ± 0.063
ND
3.51 ± 0.46
ND
2.51 ± 0.12
Albuminuria (mg/24 h)
ND
163.6 ± 18.89
ND
508.7 ± 48.44
ND
294.3 ± 21.20
Urinary NAG (U/24 h)
ND
0.098 ± 0.031
ND
0.758 ± 0.08
ND
0.432 ± 0.05
Oxidized proteins in ctx (DNPH nmol/mg protein)
2.57 ± 0.69
0.10 ± 0.01
3.75 ± 0.70
974.3 ± 53
3.27 ± 0.52
745.9 ± 51.19
Oxidized proteins in med (DNPH nmol/mg protein)
11.37 ± 3.8
18.78 ± 5.51
9.17 ± 3.4
179.8 ± 24.95
8.62 ± 2.9
166.4 ± 13.65
Lipid peroxidation in ctx (4-HNE nmol/mg protein)
0.42 ± 0.18
0.22 ± 0.1
0.90 ± 0.28
5.51 ± 0.23
0.73 ± 0.3
3.39 ± 0.24
Lipid peroxidation in med (4-HNE nmol/mg protein)
0.48 ± 0.22
0.022 ± 0.005
1.0 ± 0.18
3.44 ± 0.55
1.34 ± 0.38
1.91 ± 0.08
C: control group, D: group with diabetes, DMMF: group with diabetes treated with mycophenolate mofetil, GFR: glomerular filtration rate, NAG: N-acetyl -D-glucosaminidase, ND: not determined, ctx: renal cortex, med: renal medulla, DNPH: dinitrophenylhydrazone, and 4-HNE: 4-hydroxynonenal. Data are mean SEM of 8 animals in each group. versus control; versus D.
