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Oxidative Medicine and Cellular Longevity
Volume 2016, Article ID 4710326, 17 pages
http://dx.doi.org/10.1155/2016/4710326
Research Article

Equine Metabolic Syndrome Affects Viability, Senescence, and Stress Factors of Equine Adipose-Derived Mesenchymal Stromal Stem Cells: New Insight into EqASCs Isolated from EMS Horses in the Context of Their Aging

1Electron Microscopy Laboratory, Wroclaw University of Environmental and Life Sciences, Kozuchowska 5b, 51-631 Wroclaw, Poland
2Wroclaw Research Centre EIT+, 54-066 Wroclaw, Poland

Received 24 June 2015; Revised 31 July 2015; Accepted 10 August 2015

Academic Editor: Gabriele Saretzki

Copyright © 2016 Krzysztof Marycz et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Linked References

  1. J. M. Friedman, “Obesity in the new millennium,” Nature, vol. 404, no. 6778, pp. 632–634, 2000. View at Google Scholar · View at Scopus
  2. L. M. Pérez, A. Bernal, B. de Lucas et al., “Altered metabolic and stemness capacity of adipose tissue-derived stem cells from obese mouse and human,” PLoS One, vol. 10, no. 4, Article ID e0123397, 2015. View at Publisher · View at Google Scholar
  3. World Health Organization, “WHO Report burden: mortality, morbidity and risk factors,” WHO Rport, Global Status Report on NCDs, 2010. View at Google Scholar
  4. K. Basinska, K. Marycz, A. Śmieszek, and J. Nicpoń, “The production and distribution of IL-6 and TNF-α in subcutaneous adipose tissue and their correlation with serum concentrations in Welsh ponies with equine metabolic syndrome,” Journal of Veterinary Science, vol. 16, no. 1, pp. 113–120, 2015. View at Publisher · View at Google Scholar
  5. N. Frank, “Equine metabolic syndrome,” Veterinary Clinics of North America—Equine Practice, vol. 27, no. 1, pp. 73–92, 2011. View at Publisher · View at Google Scholar · View at Scopus
  6. P. C. Baer, “Adipose-derived mesenchymal stromal/stem cells: an update on their phenotype in vivo and in vitro,” World Journal of Stem Cells, vol. 6, no. 3, pp. 256–265, 2014. View at Publisher · View at Google Scholar
  7. B. Puissant, C. Barreau, P. Bourin et al., “Immunomodulatory effect of human adipose tissue-derived adult stem cells: comparison with bone marrow mesenchymal stem cells,” British Journal of Haematology, vol. 129, no. 1, pp. 118–129, 2005. View at Publisher · View at Google Scholar · View at Scopus
  8. R. C. Rennert, M. Sorkin, M. Januszyk et al., “Diabetes impairs the angiogenic potential of adipose-derived stem cells by selectively depleting cellular subpopulations,” Stem Cell Research and Therapy, vol. 5, no. 3, pp. 79–90, 2014. View at Publisher · View at Google Scholar · View at Scopus
  9. J. L. Martindale and N. J. Holbrook, “Cellular response to oxidative stress: signaling for suicide and survival,” Journal of Cellular Physiology, vol. 192, no. 1, pp. 1–15, 2002. View at Publisher · View at Google Scholar · View at Scopus
  10. M. Dominici, K. Le Blanc, I. Mueller et al., “Minimal criteria for defining multipotent mesenchymal stromal cells. The International Society for Cellular Therapy position statement,” Cytotherapy, vol. 8, no. 4, pp. 315–317, 2006. View at Publisher · View at Google Scholar · View at Scopus
  11. J. Grzesiak, K. Marycz, J. Czogala, K. Wrzeszcz, and J. Nicpoń, “Comparison of behavior, morphology and morphometry of equine and canine adipose derived mesenchymal stem cells in culture,” International Journal of Morphology, vol. 29, no. 3, pp. 1012–1017, 2011. View at Publisher · View at Google Scholar · View at Scopus
  12. K. Marycz, A. Śmieszek, J. Grzesiak, A. Donesz-Sikorska, and J. Krzak-Roś, “Application of bone marrow and adipose-derived mesenchymal stem cells for testing the biocompatibility of metal-based biomaterials functionalized with ascorbic acid,” Biomedical Materials, vol. 8, no. 6, Article ID 065004, 2013. View at Publisher · View at Google Scholar · View at Scopus
  13. V. Roth, http://www.doubling-time.com/compute.php.
  14. M. Marędziak, K. Marycz, A. Śmieszek, D. Lewandowski, and N. Y. Toker, “The influence of static magnetic fields on canine and equine mesenchymal stem cells derived from adipose tissue,” In Vitro Cellular & Developmental Biology—Animal, vol. 50, no. 6, pp. 562–571, 2014. View at Publisher · View at Google Scholar · View at Scopus
  15. A. Śmieszek, A. Czyrek, K. Basinska et al., “Effect of metformin on viability, morphology, and ultrastructure of mouse bone marrow-derived multipotent mesenchymal stromal cells and balb/3T3 embryonic fibroblast cell line,” BioMed Research International, vol. 2015, Article ID 769402, 14 pages, 2015. View at Publisher · View at Google Scholar
  16. P. Chomczynski and N. Sacchi, “Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction,” Analytical Biochemistry, vol. 162, no. 1, pp. 156–159, 1987. View at Publisher · View at Google Scholar · View at Scopus
  17. C. McGregor-Argo, “Appraising the portly pony: body condition and adiposity,” Veterinary Journal, vol. 179, no. 2, pp. 158–160, 2009. View at Publisher · View at Google Scholar · View at Scopus
  18. C. A. Wyse, K. A. McNie, V. J. Tannahil, J. K. Murray, and S. Love, “Prevalence of obesity in riding horses in Scotland,” Veterinary Record, vol. 162, no. 18, pp. 590–591, 2008. View at Publisher · View at Google Scholar · View at Scopus
  19. Z. Kočí, K. Turnovcová, M. Dubský et al., “Characterization of human adipose tissue-derived stromal cells isolated from diabetic patient's distal limbs with critical ischemia,” Cell Biochemistry and Function, vol. 32, no. 7, pp. 597–604, 2014. View at Publisher · View at Google Scholar · View at Scopus
  20. C.-L. Wu, B. O. Diekman, D. Jain, and F. Guilak, “Diet-induced obesity alters the differentiation potential of stem cells isolated from bone marrow, adipose tissue and infrapatellar fat pad: the effects of free fatty acids,” International Journal of Obesity, vol. 37, no. 8, pp. 1079–1087, 2013. View at Publisher · View at Google Scholar · View at Scopus
  21. S. P. Bruder, N. Jaiswal, and S. E. Haynesworth, “Growth kinetics, self-renewal, and the osteogenic potential of purified human mesenchymal stem cells during extensive subcultivation and following cryopreservation,” Journal of Cellular Biochemistry, vol. 64, no. 2, pp. 278–294, 1997. View at Publisher · View at Google Scholar · View at Scopus
  22. W. Zhang, J. Li, K. Suzuki et al., “A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging,” Science, vol. 348, no. 6239, pp. 1160–1163, 2015. View at Publisher · View at Google Scholar
  23. L. A. Kirshenbaum and P. K. Singal, “Antioxidant changes in heart hypertrophy: significance during hypoxia-reoxygenation injury,” Canadian Journal of Physiology and Pharmacology, vol. 70, no. 10, pp. 1330–1335, 1992. View at Publisher · View at Google Scholar · View at Scopus
  24. J. M. McCord, “Superoxide radical: controversies, contradictions, and paradoxes,” Proceedings of the Society for Experimental Biology and Medicine, vol. 209, no. 2, pp. 112–117, 1995. View at Publisher · View at Google Scholar · View at Scopus
  25. H. Chen and D. C. Chan, “Emerging functions of mammalian mitochondrial fusion and fission,” Human Molecular Genetics, vol. 14, supplement 2, pp. R283–R289, 2005. View at Publisher · View at Google Scholar · View at Scopus
  26. S. A. Detmer and D. C. Chan, “Functions and dysfunctions of mitochondrial dynamics,” Nature Reviews Molecular Cell Biology, vol. 8, no. 11, pp. 870–879, 2007. View at Publisher · View at Google Scholar · View at Scopus
  27. Q. Wang, A. I. Frolova, S. Purcell et al., “Mitochondrial dysfunction and apoptosis in cumulus cells of type I diabetic mice,” PLoS ONE, vol. 5, no. 12, Article ID e15901, 2010. View at Publisher · View at Google Scholar · View at Scopus
  28. A. Stolzing, S. Sethe, and A. M. Scutt, “Stressed stem cells: temperature response in aged mesenchymal stem cells,” Stem Cells and Development, vol. 15, no. 4, pp. 478–487, 2006. View at Publisher · View at Google Scholar · View at Scopus
  29. J. F. Passos, G. Nelson, C. Wang et al., “Feedback between p21 and reactive oxygen production is necessary for cell senescence,” Molecular Systems Biology, vol. 6, article 347, 2010. View at Publisher · View at Google Scholar · View at Scopus
  30. D. R. Green and J. C. Reed, “Mitochondria and apoptosis,” Science, vol. 281, no. 5381, pp. 1309–1312, 1998. View at Publisher · View at Google Scholar · View at Scopus
  31. J. Yang, X. Liu, K. Bhalla et al., “Prevention of apoptosis by Bcl-2: release of cytochrome c from mitochondria blocked,” Science, vol. 275, no. 5303, pp. 1129–1132, 1997. View at Google Scholar
  32. D. Bonnefont-Rousselot, “Glucose and reactive oxygen species,” Current Opinion in Clinical Nutrition & Metabolic Care, vol. 5, no. 5, pp. 561–568, 2002. View at Google Scholar
  33. F. Giacco and M. Brownlee, “Oxidative stress and diabetic complications,” Circulation Research, vol. 107, no. 9, pp. 1058–1070, 2010. View at Publisher · View at Google Scholar · View at Scopus