Research Article
The Combination of α-Tocopheryl Succinate and Sodium Selenite on Breast Cancer: A Merit or a Demerit?
Figure 5
Effect of 60 μM α-TOS, 2 μM SSe, and their combination on beclin-1 protein level following (a) 16 hours and (b) 48 hours and on LC3B protein level following (c) 16 hours and (d) 48 hours of exposure in MCF7 cells using western blot technique and (e) effect of CQ (50 μM) on IC50 of SSe after 48 hours. Acridine orange stained MCF7 cells after exposure to 60 μM α-TOS, 2 μM SSe, and their combination after (f) 48 hours [original magnification (objective lens (20x))]. Cells with acidic vesicular organelles (AVOs) can be visualized by their orange fluorescence (pointed by black arrows) in the cytoplasm, whereas cells without AVOs are marked by red arrows. Western blot results are expressed as means ± SD of 3 independent experiments (). Statistical significance of results was analyzed by one-way ANOVA using Tukey’s multiple comparison test. “A” significantly different from the respective control at ; “B” significantly different from respective α-TOS at ; “C” significantly different from respective SSe at .
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