Astragalus Polysaccharide Suppresses 6-Hydroxydopamine-Induced Neurotoxicity in Caenorhabditis elegans

Figure 1

Effect of astragalan on 6-OHDA-induced degeneration of dopaminergic neurons in C. elegans. (a) Representative fluorescence images of Pdat-1::GFP in dopaminergic neurons were taken from the transgenic C. elegans strain BZ555. The nematodes were exposed to 50 mM 6-OHDA for 1 h prior to treatment with astragalan (2.0 mg/mL) or EGCG (0.05 mM). Scale bars, 20 μm. (b) Graphical presentation for fluorescence intensity of Pdat-1::GFP in dopaminergic neurons of the nematodes treated with or without astragalan at the indicated concentrations (0.5–4.0 mg/mL). Results are presented as mean ± SEM of three independent experiments. versus 6-OHDA-exposed nematodes.

(a)

(b)