JC induces apoptosis. (a) BEL-7402 HCC cells were treated with indicated concentrations of JC for 48 hours and then subjected to the MTT cell viability assay. (b, c) Detection of apoptosis in JC-induced HCC cells by DAPI staining. HepG2 cells were treated with DMSO or 8 μg/mL of JC for the indicated times (b). BEl-7402 cells were treated with 8.7 μg/mL of JC for the indicated times (c). Then, the cells were fixed and stained with DAPI. Arrows are used to indicate apoptotic bodies in apoptotic HCC cells. (d) DNA microarray analysis of the genes enriched in cell death, programmed death, and apoptosis after HepG2 cells were treated with JC for 10 hours. (e) GO classification for upregulated genes after HepG2 cells were treated with JC for 4 hours. (f) Clustering analysis of genes that can positively regulate Akt signaling after HepG2 cells were treated with JC for 4 hours.