Protective Role of Dietary Curcumin in the Prevention of the Oxidative Stress Induced by Chronic Alcohol with respect to Hepatic Injury and Antiatherogenic Markers

Figure 6

Effect of chronic alcohol, ω-3 PUFA, and curcumin on 4-HNE protein adducts levels (×200) in liver samples of various groups. The animals in the indicated groups () were pair-fed their respective Lieber-DeCarli alcohol containing liquid diets supplemented with the indicated concentration of curcumin for 8 weeks after which the animals were killed and each rat liver sample was paraffin sectioned and stained with 4-HNE specific antibody. (1) Representative photomicrographs of immunohistochemically stained 4-HNE protein adducts (scale bars: 200 μm) in liver sections of following groups shown in (a) LFO; (b) LFOE; (c) LFOEC; (d) HFO; (e) HFOE; and (f) HFOEC and (g). Bar graph shows the % of pixel values of brown color representing 4-HNE specific antibody reactivity from four randomly selected regions per section of each group () measured using AxioVision Rel.4.8.2 software. The data are Means ± SEM. Statistical significance of variance was calculated using t-test with Bonferroni correction; compared to all groups except HFOEC group; compared to HFOE group.

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