Low-Dose Methylmercury-Induced Apoptosis and Mitochondrial DNA Mutation in Human Embryonic Neural Progenitor Cells

Figure 2

MeHg-induced apoptosis in hNPCs. (a) Cells were stained with Annexin V and PI. Dot plots showed representative staining by flow cytometry, showing the existence of 4 cellular populations: AnnV−/PI−, AnnV+/PI−, AnnV−/PI+, and AnnV+/PI+ (% of each population for all three conditions is provided). (b) The degree ofapoptosis was shown as (or calculated by) the percentage of Annexin V-positive cells in cells treated with MeHg. All experiments were repeated three times (technical triplicates) with biological triplicates (). Bar graphs show mean ± SEM ().

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