Polydatin Attenuates H₂O₂-Induced Oxidative Stress via PKC Pathway

Figure 2

Effects of PD on LDH, ROS, GSH-Px, and SOD in OS-injured HUVECs. HUVECs were treated with H₂O₂ (400 μM) alone or with PD (3 μg/mL). (a) The release of LDH from HUVECs was detected using an assay kit. (b) The intensity of DCFH fluorescence was measured to evaluate the intracellular ROS level. (c) The intracellular GSH-Px level of HUVECs. (d) The intracellular SOD level of HUVECs. The results are expressed as mean ± SEM, = 3 (, compared to control group, , , compared to H₂O₂ group). Significant differences between groups were analyzed with one-way ANOVA.

(a)

(b)

(c)

(d)