Protective Effects of D-Penicillamine on Catecholamine-Induced Myocardial Injury

Figure 2

Overview of the protocols for cell experiments using the H9c2 cardiomyoblast cell line. Catecholamines (CA-ISO or epinephrine) were added to H9c2 cells either as freshly prepared solutions (a and b) or after 24 h preincubation in the cell-culture medium at 37 °C (oxidized oxCA) (c–e). D-PA was added either at the beginning of 24 h cellular experiments (b and d) or at the start of 24 h catecholamine preincubation before the 24 h cellular experiments (e).