Lung Neutrophilia in Myeloperoxidase Deficient Mice during the Course of Acute Pulmonary Inflammation

Figure 3

Total blood neutrophil count and surface expression of CD11b by neutrophils during the course of acute lung inflammation. (a) Total blood count of neutrophils was determined from total blood leukocytes count and their relative differentiation count in blood samples taken at various times (8 h, 24 h, 48 h, and 72 h) after instillation of LPS (0.3 mg/kg) or PBS (control at time 0 h) in both WT (black bars) and MPO KO (grey bars) mice. (b) Expression of surface CD11b receptor on blood neutrophils was determined by flow cytometer with a use of fluorescent labeled anti-CD11b monoclonal antibodies in blood samples described above. Results are expressed as relative fluorescence units (RFU). Values represent mean ± SEM from 8–10 mice.

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