Lung Neutrophilia in Myeloperoxidase Deficient Mice during the Course of Acute Pulmonary Inflammation

Figure 9

Determination of caspase 3 activity and DNA fragmentation. (a) Freshly isolated nucleated cells or cells 5 h after isolation ( cells/mL) from BALF of WT and MPO KO mice treated for 36 h by intranasal application of LPS (0.3 mg/kg) were lysed and caspase 3 activity was determined by enzymatic assay. Cells treated with anti-Fas antibody were used as a positive control. Values represent mean ± SEM from 8–10 mice with significant difference between WT or MPO KO mice and positive control (). (b) DNA extracted from freshly isolated cells (0 h) or cells incubated in vitro for 5 h (5 h). Cells were isolated from BALF of WT mice treated for 36 h by intranasal application of LPS (0.3 mg/kg). UV-irradiated BALF cells were used as a positive control.

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