Role of TFEB Mediated Autophagy, Oxidative Stress, Inflammation, and Cell Death in Endotoxin Induced Myocardial Toxicity of Young and Aged Mice

Figure 7

Effect of LPS in young and aged mice on cardiac autophagy. Markers of autophagy LC3 with loading control GAPDH were determined by western blot analyses (a). Both nuclear and cytoplasmic fractions of TFEB, along with nuclear specific marker Histone H3 and cytoplasmic marker GAPDH, were determined by western blot experiments (b). LAMP-1, downstream effector of TFEB and involved in lysosomal biogenesis, was also analyzed (c). Significant autophagy response in LPS treated mice was observed. However, LPS induced cardiac autophagic response in aged mice was significantly lower than young ones.

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