Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2/STAT3 Signaling

Figure 2

Notch signal blockade aggravates burn-induced cardiomyocyte apoptosis in vitro. Rat cardiomyocytes were challenged with burn serum in vitro in the presence of DMSO or GSI. Apoptotic cells were stained by AO/EB staining 12 hours after challenge (a, magnification ×200) and were quantified (b). Cells were collected to determine the expression of caspase-3 protein expression (c). Cell supernatants in (c) were collected and LDH production (d) was assessed. The values presented are the mean ± SEM ( per group). .

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