Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2/STAT3 Signaling

Figure 5

ROS scavenger alleviates burn-induced myocardial injury aggravated by Notch signal deficiency. Rat cardiomyocytes were challenged with burn serum in vitro in the presence of DMSO or GSI, with or without the ROS scavenger, NAC. ROS were examined by way of FACS (a) and were quantified by way of mean fluorescence intensity (b). (c) Apoptotic cells were stained by AO/EB staining. (d) Quantitative comparison of apoptotic cells upon AO/EB staining in (c). Cell supernatants were collected and LDH production (e) was assessed. The values presented are the mean ± SEM ( per group). .

(a)

(b)

(c)

(d)

(e)