Notch1 Pathway Protects against Burn-Induced Myocardial Injury by Repressing Reactive Oxygen Species Production through JAK2/STAT3 Signaling

Figure 7

Inhibition of JAK2/STAT3 signaling preferentially aggravated myocardial injury after burn. Rat cardiomyocytes were challenged with burn serum in vitro in the presence of AG490, with or without GSI. JAK2/STAT3 (a, b) and MnSOD (c) expression was determined by western blot in cardiomyocytes. ROS were examined by way of FACS (d) and were quantified by way of mean fluorescence intensity (e). (f) Apoptotic cells were stained by AO/EB staining. (g) Quantitative comparison of apoptotic cells upon AO/EB staining in (f). The values presented are the mean ± SEM ( per group). . NS, not significant.

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