Oxidative Medicine and Cellular Longevity / 2016 / Article / Tab 2 / Research Article
The Cellular Response to Oxidatively Induced DNA Damage and Polymorphism of Some DNA Repair Genes Associated with Clinicopathological Features of Bladder Cancer Table 2 Characteristics of allelic variants and some conditions for their detection.
Gene polymorphisms Primer sequences Restriction enzyme PCR products (bp) Nucleotide excision repair ERCC2/XPD Asp312Asn rs1799793(F) 5′-CTG TTG GTG GGT GCC CGT ATC TGT TGG TCT-3′ (R) 5′-TAA TAT CGG GGC TCA CCC TGC AGC ACT TCC T-3′ StyI Asp/Asp: 507 + 244; Asp/Asn: 507 + 474 + 244 + 33; Asn/Asn: 474 + 244 + 33 ERCC2/XPD Lys751Gln rs13181(F) 5′-GCC CGC TCT GGA TTA TAC G-3′ (R) 5′-CTA TCA TCT CCT GGC CCC C-3′ Pst I Lys/Lys: 290 + 146; Lys/Gln: 290 + 127 + 146; Gln/Gln: 227 + 146 ERCC6/CSB Met1097Val rs2228526(F) 5′-CCT GCT T CT AAC ATA TCT GT-3′ (R) 5′-AAT CAC TGA CAA CTC TTC TG-3′ Nla III Met/Met: 123 + 78; Met/Val: 201 + 123 + 78; Val/Val: 201 ERCC6/CSB Gly399Asp rs2228528(F) 5′-TGA AGA GTC TGA GTA TTT CC-3′ (R) 5′-ATC TTC ATC TCC ATC ATC TC-3′ RsaI Gly/Gly: 180 + 91; Gly/Asp: 271 + 180 + 91; Asp/Asp: 271 Base excision repair XRCC1 Arg399Gln rs25487(F) 5′-GGA CTG TCA CCG CAT GCG TCG G-3′ (R) 5′-GGC TGG GAC CAC CTG TGT T-3′ MspI Arg/Arg: 115 + 34; Arg/Gln: 149 + 115 + 34; Gln/Gln: 149 OGG1 Ser326Cys rs1052133
(F) 5′-CTG TTC AGT GCC GAC CTG CGC CGA-3′ (R) 5′-ATC TTG TTG TGC AAA CTG AC-3′ MboI Ser/Ser: 224 + 23; Ser/Cys: 247 + 224 + 23; Cys/Cys: 247