Conservation of the Nrf2-Mediated Gene Regulation of Proteasome Subunits and Glucose Metabolism in Zebrafish

Figure 5

The expression of other candidate genes for zebrafish Nrf2 targets. (a) The expression of the indicated genes in 8 hpf wild-type embryos injected with or without 100 pg of nrf2a mRNA at the 1-cell stage was analyzed by a real-time qPCR. Asterisks denote statistical significance (Control versus nrf2a overexpression, and ; Student’s t-test, for each group). (b) The expression of the indicated genes in 4 dpf wild-type or nrf2a^fh318/fh318 mutant larvae treated with or without 100 μM DEM for 6 h was analyzed by a real-time qPCR. Asterisks and hash marks denote statistical significance (DEM+ versus DEM−, and ; wild-type versus nrf2a^fh318/fh318, and ; Student’s t-test, for each group).

(a)

(b)