The Effect of Leonurus sibiricus Plant Extracts on Stimulating Repair and Protective Activity against Oxidative DNA Damage in CHO Cells and Content of Phenolic Compounds

Figure 4

The SOD2 (a), CAT (b), GPx (c), and -actin mRNA levels in the CHO cell line, incubated in the presence of 0.5 mg/mL AP, R in vitro plant and AP, R in vivo plant extracts of L. sibiricus for 24 h. -actin was used as an internal control for integrity and equal amount of cDNA used in each PCR. Representative gels from three independent experiments are shown. Before treatment with plant extracts, the cells were exposed to H₂O₂ at 75 μM for 15 min. The bar graph shows a semiquantitative comparison of the SOD2, CAT,and GPx to -actin optical density ratio. as compared to AP, R in vitro plant and AP, R in vivo plant extracts with control the appropriate time of incubation.