The Effect of Leonurus sibiricus Plant Extracts on Stimulating Repair and Protective Activity against Oxidative DNA Damage in CHO Cells and Content of Phenolic Compounds

Table 2

Phenolic compounds identified in L. sibiricus extracts using HPLC-ESI-MS/MS method.

Peak number	Phenolic compounds	max (nm)	RT (min)	Molecular (precursor) ion M-H ()	Main fragment ions ()
1	4-Hydroxybenzoic acid	211, 253	15.26	137	93 [M-CO2-H]^−
2	(+)-Catechin; (flavan-3-ol)	230, 278	15.90	289	245 [M-CH2-CH-OH-H]^−
3	Chlorogenic acid	217, 241; 294, 231	18.13	353	191 [QA-H]^−; 179 [CA-H]^−; 173 [QA-H2O-H]^−; 135 [CA-CO2-H]^−
4	Caffeic acid	215, 241; 291, 319	18.81	179	135 [M-CO2-H]^−; 107 [M-CO2-CO-H]^−; 89 [M-CO2-CO-H2O-H]^−
5	-Coumaric acid	207, 226; 291, 307	22.67	163	119 [M-CO2-H]^−
6	Ferulic acid	214, 239; 291, 325	23.88	193	179 [M-CH2-H]^−; 149 [M-CO2-H]^−; 135 [M-CO2-CH2-H]^−
7	Rutin	255, 265; 294, 352	27.89	609	301 [M-rutinose-H]^−
8	Quercetin	253, 268; 297, 368	33.58	301	271 (M-CH2O-H]^−; 179 [M--H]^−; 151 [M--CO-H]^−
9	Verbascoside	237, 296, 330	29.63	623	669 [M+HCOO]^−; 461 [M-H-CA]^−; 161 [CA-H2O-H]^−

CA: caffeic acid; QA: quinic acid.