The Effect of Leonurus sibiricus Plant Extracts on Stimulating Repair and Protective Activity against Oxidative DNA Damage in CHO Cells and Content of Phenolic Compounds

Table 3

Contents of phenolic compounds in extracts from aerial part and roots of micropropagated and seed-derived plants of L. sibiricus.

Number	Phenolic compounds	AP in vitro; mg/g DW	R in vitro; mg/g DW	AP in vivo; mg/g DW	R in vivo; mg/g DW
1	4-Hydroxybenzoic acid	0,090 ± 0,004	0,041 ± 0,0017	0,16 ± 0,0073	0,004 ± 0,0001
2	(+)-Catechin	0,071 ± 0,0033	0,1 ± 0,004	0,184 ± 0,0083	0,054 ± 0,0024
3	Chlorogenic acid	4,912 ± 0,246	6,532 ± 0,306	3,125 ± 0,054	4,380 ± 0,187
4	Caffeic acid	1,235 ± 0,060	5,480 ± 0,244	1,361 ± 0,066	3,222 ± 0,127
5	-Coumaric acid	—	0,011 ± 0,0004	0,019 ± 0,0006	—
6	Ferulic acid	0,034 ± 0,0152	0,028 ± 0,0011	0,027 ± 0,0010	0,020 ± 0,0006
7	Rutin	0,018 ± 0,0008	0,502 ± 0,022	0,014 ± 0,0005	0,172 ± 0,0051
8	Quercetin	9,712 ± 0,372	—	2,848 ± 0,108	—
9	Verbascoside	0,188 ± 0,008^a	0,373 ± 0,013^c	0,180 ± 0,007^a	0,255 ± 0,002^b
10	Sum of phenolic acids	6,273 ± 0,301^b	12,093 ± 0,568^c	4,694 ± 0,192^a	7,628 ± 0,299^b
11	Sum of flavonoids	9,730 ± 0,370^d	0,502 ± 0,022^b	2,862 ± 0,107^c	0,172 ± 0,0051^a

AP and R in vitro plant extracts: 80% aqueous methanol extracts of aerial parts and roots of micropropagated plants.
AP and R in vivo plant extracts: 80% aqueous methanol extracts of aerial parts and roots of seed-derived plants.
Different superscript letters within the rows indicate significant differences in the mean values at (one-way ANOVA by the Mann-Whitney test).