Antioxidant and DNA Repair Stimulating Effect of Extracts from Transformed and Normal Roots of Rhaponticum carthamoides against Induced Oxidative Stress and DNA Damage in CHO Cells

Figure 3

Oxidative DNA damage as percent of DNA in the comet tails in CHO cells. DNA damage was induced by H₂O₂ at a concentration of 50 μM at 4°C on ice. After treatment with H₂O₂, CHO cells were treated for 24 h with 1.0 mg/mL of R. carthamoides TR and NR extracts. as compared to TR and NR extract with H₂O₂. The number of cells scored was 100. Data is expressed as means ± SE of three independent experiments. Alkaline version: strand breaks and alkali-labile sites; Nth: oxidative DNA damage recognized by Nth; hOGG1: oxidative DNA damage recognized by hOGG1.