Antioxidant and DNA Repair Stimulating Effect of Extracts from Transformed and Normal Roots of Rhaponticum carthamoides against Induced Oxidative Stress and DNA Damage in CHO Cells

Figure 6

The SOD2, CAT, and -actin mRNA levels in the CHO cell line, incubated in the presence of 1.0 mg/mL R. carthamoides TR and NR extracts (a) for 24 h. The expression of each target gene was standardized with an internal control gene (β-actin) and represented as a ratio. Representative gels from three independent experiments are shown. Before treatment with plant extracts, the cells were exposed to H₂O₂ at 50 μM for 10 min. The bar graph shows a semiquantitative comparison of SOD2 (b) and CAT (c) to -actin optical density ratio. Results were represented as means ± SE from three independent experiments. as compared to TR and NR extract with control (cells incubated with PBS). Control 24 h: cells incubated for 24 h with PBS; H₂O₂: cells incubated with H₂O₂; NR 24 h: cells incubated for 24 h with NR extract; TR 24 h: cells incubated for 24 h with TR extract; H₂O₂ + NR 24 h: cells treated with H₂O₂ before 24 h incubation with NR extract; H₂O₂ + TR 24 h: cells treated with H₂O₂ before 24 h incubation with TR extract.

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