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Oxidative Medicine and Cellular Longevity
Volume 2016 (2016), Article ID 5818479, 8 pages
http://dx.doi.org/10.1155/2016/5818479
Research Article

Antioxidant and Anti-Inflammatory Effects of Coenzyme Q10 on L-Arginine-Induced Acute Pancreatitis in Rat

1Department of Surgery, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
2Students’ Research Committee, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
3Young Researchers and Elite Club, Islamic Azad University, Tehran Medical Sciences Branch, Tehran, Iran
4Student Research Committee, Shiraz University of Medical Sciences, Shiraz, Iran
5Students’ Research Committee, Baqiyatallah University of Medical Sciences, Tehran, Iran

Received 12 January 2016; Revised 10 March 2016; Accepted 22 March 2016

Academic Editor: Giuseppe Cirillo

Copyright © 2016 Seyed Abbas Mirmalek et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

This study was aimed at evaluating the protective effect of coenzyme Q10 on L-arginine-induced acute pancreatitis in rats regarding biomarkers and morphologic changes. Thirty-two male Sprague-Dawley rats were divided into 4 equal groups. Control group received intraperitoneal normal saline, while in sham and experimental groups 1 and 2 pancreatitis was induced with L-arginine. E1 and E2 groups were treated with a single dose of 100 and 200 mg/kg Q10, respectively. Serum lipase and amylase, along with pancreas IL-10, IL-1β, and TNF-α, were measured. For evaluation of oxidative stress, pancreatic superoxide dismutase (SOD), glutathione (GSH), malondialdehyde (MDA), and myeloperoxidase (MPO) were assessed. Histopathological examination for morphologic investigation was conducted. Serum amylase and lipase, as well as TNF-α and IL-1β cytokines, reverted with administration of Q10 in consistence with dosage. In contrast, Q10 assisted in boosting of IL-10 with higher dosage (200 mg/kg). A similar pattern for oxidative stress markers was noticed. Both MDA and MPO levels declined with increased dosage, contrary to elevation of SOD and GSH. Histopathology was in favor of protective effects of Q10. Our findings proved the amelioration of pancreatic injury by Q10, which suggest the anti-inflammatory and antioxidant property of Q10 and its potential therapeutic role.