Oxidative Medicine and Cellular Longevity

Research Article

Identification of Redox and Glucose-Dependent Txnip Protein Interactions

Table 1

List of proteins identified by BirA-Txnip.


Protein name¹	Gene ID	UniProt	Localization²	Molecular function³	C247S

Src substrate cortactin	CTTN	Q14247	Plasma membrane	Actin polymerization	Yes

Filamin-A, isoform 2	FLNA	Q60FE6	Cytoplasm	Actin polymerization	No

4F2 cell-surface antigen heavy chain, isoform 2	SLC3A2	P08195	Plasma membrane	Amino acid transport	Yes

Heat shock protein HSP 90-beta	HSP90AB1	P08238	Cytoplasm	Chaperone	No

Heat shock 70 kDa protein 1A/1B	HSPA1A/1B	P0DMV8	Cytoplasm	Chaperone	Yes

Heat shock 70 kDa protein 13	HSPA13	P48723	ER	Chaperone	Yes

DnaJ homolog subfamily C member 7	DNAJC7	Q99615	Cytoplasm	Chaperone	Yes

Heat shock cognate 71 kDa protein	HSPA8	P11142	Cytoplasm, nucleus	Chaperone	Yes

Stress-induced phosphoprotein 1	STIP1	P31948	Cytoplasm, nucleus	Chaperone	Yes

JmjC domain-containing histone demethylation protein 2C	JMJD1C	B7ZLC8	Nucleus	Chromatin structure	No

TOX high mobility group box family member 4	TOX4	O94842	Nucleus	Chromatin structure	Yes

YEATS domain-containing protein 2	YEATS2	Q9ULM3	Nucleus	Chromatin structure	Yes

Double-strand break repair protein MRE11A	MRE11A	B3KTC7	Nucleus	DNA repair	No

Poly ADP-ribose polymerase 1	PARP1	P09874	Nucleus	DNA repair	No

Epidermal growth factor receptor substrate 15-like 1	EPS15L1	A5PKY0	Vesicles	Metal binding	No

Nuclear mitotic apparatus protein 1	NUMA1	Q14980	Nucleus	Mitotic spindle formation	Yes

TPR nucleoprotein	TPR	P12270	Nucleus	Nuclear transport	No

E3 ubiquitin-protein ligase Itchy homolog	ITCH	Q96J02	Cytoplasm	Proteolysis	No

E2 ubiquitin-conjugating enzyme	UBE2O	Q9C0C9	Nucleus	Proteolysis	Yes

Thioredoxin-like protein 1	TXNL1	O43396	Cytoplasm	Redox	Yes

Neuroblast differentiation-associated protein AHNAK	AHNAK	Q09666	Nucleus	RNA binding	No

Nucleolar protein 58	NOP58	Q9Y2X3	Nucleus	RNA binding	No

U4/U6.U5 tri-snRNP-associated protein 1	SART1	O43290	Nucleus	RNA binding	Yes

Activity-dependent neuroprotector homeobox protein	ADNP	Q9H2P0	Nucleus	Transcription	Yes

SAP30-binding protein	SAP30BP	Q9UHR5	Nucleus	Transcription	No

Sex comb on midleg-like protein 2	SCML2	H0Y6S1	Nucleus	Transcription	No

Transcription intermediary factor 1-beta	TRIM28	Q13263	Nucleus	Transcription	Yes

Transcriptional regulator Kaiso	ZBTB33	Q86T24	Nucleus	Transcription	No

Eukaryotic translation initiation factor 5	EIF5	P55010	Cytoplasm	Translation	Yes

cDNA FLJ56180 fis	N/A	Q6ZNN8	Unknown	Unknown	No

UPF0428 protein CXorf56	CXorf56	Q9H5V9	Nucleus	Unknown	No

Proteins were identified on at least 2 of 3 biological replicates.
²Primary localization based on information from UniProt and The Human Protein Atlas.
³Based on information from UniProt and NCBI:Gene reports.