Figure 5: Treatment with morin attenuated intracellular ROS production induced by TNF-α. (a) Fluorescence-activated cell sorting profile of ROS generation by flow cytometry. Summary of the average ROS production by BECs treated with morin (10 μM) or NAC (1 and 10 mM) after TNF-α (10 ng/mL) stimulation in three independent experiments. (b) DCFH-DA fluorescence (green) imaging of ROS in BECs evaluated with a laser scanning confocal microscope. Scale bar: 50 μm. Values represented as mean ± SD of at least four independent experiments performed in triplicate. compared with the control, and compared with the TNF-α group. M: morin (10 μM); T: TNF-α (10 ng/mL); N: NAC.